rabbit anti wls  (Proteintech)

Journal: Cell Death & Disease

Article Title: Targeting sorting nexin 3 to treat pulmonary fibrosis by dual modulating Wnt/β-catenin signaling

doi: 10.1038/s41419-025-08248-x

Figure Lengend Snippet: A The protein mass spectrum of Wls were shown. B Fresh lung homogenates were sparked by anti-Flag or anti-Wls for Wls or SNX3 detection in co-IP analysis. C IHC staining analysis shown the Wls expression in BLM-induced mice (Scale bar: 200 μm; n = 8 mice). D The protein level and colocalization of Wls with SNX3 in Snx3-cTg mice were detected by IF staining analysis (Scale bar: 100 μm, n = 8 mice). E The protein level of Wls were detected by IHC staining analysis in Snx3-cTg mice (Scale bar: 200 μm; n = 8 mice). F The protein level and colocalization of Wls with SNX3 in Snx3-cKO mice were detected by IF staining analysis (Scale bar: 100 μm, n = 8 mice). G The protein level of Wls in Snx3-cKO mice were detected by western blotting analysis ( n = 8 mice). H , I IF staining analysis were performed to detected the protein level and colocalization of Wls with Rab 5a and LAMP1; Scale bar: 10 μm; n = 3 experiments. J The Wls intracellular recycling and degradation flow chart. Were created with BioGDP.com K H&E staining and PSR staining in lung tissue sections were shown. Scale bar: 200 μm, n = 8 mice. L Representative images of IHC staining analysis in Snx3-cKO mice were shown; Scale bar: 200 μm, n = 8 mice. M IF staining analysis of α-SMA were shown; Scale bar: 25 μm, n = 3 experiments. N IF staining analysis of β-catenin were shown; Scale bar: 25 μm, n = 3 experiments. The data were shown as means ± SEM. * P < 0.05 vs. CTL+ Saline group or sh-NC. # P < 0.05 vs CTL + BLM group or sh-NC + TGF-β1. & P < 0.05 vs Snx3-cKO + BLM group or sh-SNX3 + TGF-β1. ns, not significant.

Article Snippet: Then, cells were blocked with PLA blocking buffer for 1 h at 37 °C and incubated with rabbit anti-Wls (Proteintech, China, #23081-1-AP), anti-CK-1α (Abcam, USA, # EPR19824 ) and mouse anti-SNX3 (Santa Cruz, USA, diluted 1:200, #sc-376667) overnight at 4 °C.

Techniques: Co-Immunoprecipitation Assay, Immunohistochemistry, Expressing, Staining, Western Blot, Saline

Journal: iScience

Article Title: AID-induced CXCL12 upregulation enhances castration-resistant prostate cancer cell metastasis by stabilizing β-catenin expression

doi: 10.1016/j.isci.2023.108523

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-WLS , Thermo Fisher Scientific , Cat# PA5-98483; RRID: AB_2813096.

Techniques: Virus, Recombinant, Reverse Transcription, Plasmid Preparation, Cloning, CCK-8 Assay, shRNA, Software

Journal: bioRxiv

Article Title: Deficient FGF signaling in the developing peripheral retina disrupts ciliary margin development and causes aniridia

doi: 10.1101/443416

Figure Lengend Snippet: A. Atoh7, a neural retinal marker indicates expansion of the ciliary margin in FGF signaling mutants. Vsx2 expression domain in the neural retina and proximal ciliary margin is also similarly ectopically pushed back into the neural retinal domain. Mitf expression in the RPE and distal ciliary margin is expanded ectopically towards the neural retinal domain. Msx1 expression in the proximal ciliary margin is noticeably lost. Wfdc1 expression from the proximal and medial ciliary margin is significantly diminished. Otx1 expression in the ciliary margin is expanded further towards the neural retina. Axin2 expression restricted normally to the distal and medial ciliary margin and a marker for Wnt signaling is expanded ectopically as well. B. Sox2, a neural retinal marker displays a graded expression in the proximal ciliary margin and is not present in the distal ciliary margin. It is noticeably absent in the proximal ciliary margin in the FGF signaling mutants. Pax6 expression is strong in the ciliary margin and weans towards the neural retina. The strong expression of Pax6 is seen expanded in FGF signaling mutants. Otx2 marks the RPE and distal ciliary margin cells. Otx2 expression is expanded into the neural retinal space. Wls expression in the RPE (which is not visible with pigmentation) and distal ciliary margin is also expanded. Cdo, a pan marker for ciliary margin is expanded towards the neural retinal space. Expression of Lef1 restricted normally to the distal and medial ciliary margin and a marker for Wnt signaling is expanded in the ectopic ciliary margin. pERK expression in the neural retina, proximal and medial ciliary margin is noticeably absent in the ciliary margin of FGF mutants. C. A comparison of Cdo, Msx1, Otx2, and Sox2 expression with Wls expression reveals the expansion of the distal ciliary margin at the expense of the proximal ciliary margin in the FGF mutants.

Article Snippet: Antibodies used were: Goat anti-Sox2 (Santa Cruz Biotechnology), Rabbit anti-Pax6 (Covance Research Products Inc.), Goat anti-Otx2 (Santa Cruz Biotechnology), Rabbit anti-Wls (Seven Hills Bioreagents), Goat anti-Cdo (R&D Systems), Rabbit anti-Lef1 (Cell Signaling Technology), Rabbit anti-pERK (Cell Signaling Technology), Goat anti-Msx1 (R&D Systems), Rabbit anti-GFP (Thermo Fisher Scientific), Chick anti-GFP (Aves Labs), Mouse anti-Brdu (Developmental Studies Hybridoma Bank) and Mouse anti-αSMA (Sigma-Aldrich).

Techniques: Marker, Expressing

Journal: bioRxiv

Article Title: Deficient FGF signaling in the developing peripheral retina disrupts ciliary margin development and causes aniridia

doi: 10.1101/443416

Figure Lengend Snippet: A. Loss of either β-catenin or Lrp5/Lrp6 in the peripheral retina resulted in similar defect in the ciliary margin. Atoh7 expression domain is pushed closer towards the distal tip of the optic cup indicating ectopic expansion of the neural retinal domain. Vsx2 expression is expanded to the distal tip of the optic cup. Mitf expression in the distal ciliary margin and Msx2 expression in the ciliary margin are lost in WNT signaling mutants. Wfdc1 and Otx1 expression is restricted to a small portion of the distal ciliary margin while Axin2 expression is completely lost from the ciliary margin. B. Stronger expression of Sox2 is seen in the distal tip of the ciliary margin in the WNT signaling mutants. The stronger expression of Pax6 in the ciliary margin is restricted to the distal tip of the optic cup. Otx2 expression from the distal ciliary margin is lost, Wls expression from the distal ciliary margin is lost and Lef1 expression from the distal and medial ciliary margin is lost. Cdo expression is restricted to a small region of the distal optic cup. pERK expression is expanded towards the distal tip of the ciliary margin.

Article Snippet: Antibodies used were: Goat anti-Sox2 (Santa Cruz Biotechnology), Rabbit anti-Pax6 (Covance Research Products Inc.), Goat anti-Otx2 (Santa Cruz Biotechnology), Rabbit anti-Wls (Seven Hills Bioreagents), Goat anti-Cdo (R&D Systems), Rabbit anti-Lef1 (Cell Signaling Technology), Rabbit anti-pERK (Cell Signaling Technology), Goat anti-Msx1 (R&D Systems), Rabbit anti-GFP (Thermo Fisher Scientific), Chick anti-GFP (Aves Labs), Mouse anti-Brdu (Developmental Studies Hybridoma Bank) and Mouse anti-αSMA (Sigma-Aldrich).

Techniques: Expressing

Journal: Behavioral neuroscience

Article Title: Low Expression of D2R and Wntless Correlates with High Motivation for Heroin

doi: 10.1037/bne0000104

Figure Lengend Snippet: Western blot of Wntless/GPR177 expression. Samples from 14 heroin self-administering rats were prepared from the prefrontal cortex (PFC). Western blots were run and probed for Wntless/GPR177. Subject identification numbers mark each lane. Bottom bands show Ponceau loading control.

Article Snippet: After destaining, membranes were incubated at room temperature for 1 hour with the following antibodies: rabbit anti-D 2 dopamine receptor (1:1,000, Millipore, Bedford, MA); mouse anti-dynamin (1:10,000, Oncogene Research Products, Cambridge, MA); rabbit anti-GPR177/WLS (1:20,000; Sigma); rabbit anti-VAP33/VAPA antisera (1:10,000, generous gift of Dr. Paul Skehel, Centre for Integrative Physiology, University of Edinburgh, Scotland).

Techniques: Western Blot, Expressing